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. 2015 Jan 19;38(3):229–235. doi: 10.14348/molcells.2015.2253

Fig. 4.

Fig. 4.

Effect of NAM on SA β-Gal activity in senescent fibroblasts. Early passage (p19) and senescent (p36.5) fibroblasts were incubated with 5 mM NAM for 1 or 3 days, and were then stained for SA β-Gal activity in situ. A group of well-stained cells were imaged in bright field. Cells at p36.5 were bigger and highly positive for SA β-Gal activity. In contrast, the NAM-treated cells were decreased in size and had a lower level of SA β-Gal activity (A). (B) Quantitative measurements by using CPRG also indicated that there was a reduction in the β-galactosidase activity in the NAM-treated senescent cells (p36.5). NAM treatment had least effect on the late-passage (p28) and early-passage cells (p19). The mean values from triplicate experiments were used to make the plot. The [*P < 0.05 (compared to the mock-treated cells (without NAM) by ANOVA test (Dunnett’s test)].