Fig. 2.

Time course of C. elegans development in the presence or absence of caffeine. N2 worms synchronized at the L1 larval stage were cultured on NGM agar plates containing 0, 10, or 30 mM caffeine, and the developmental stage of each individual was scored in 24 h intervals for 96 h at 20°C (A–D). Percent distribution of the worms at each developmental stage in each population was then plotted as a line graph at the indicated time points. The developmental time course was scored for about 900 individuals (triplicates of 300 individuals/plate) at each concentration of caffeine treatment. Average values at each time point for each developmental stage are summarized in Supplementary Table S2. L1 to L4, L1 to L4 larval stages; YA, young adult stage in which egg production had not yet started; A, adult stage in which egg production had already commenced.