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. 2015 Feb 4;38(3):251–258. doi: 10.14348/molcells.2015.2302

Fig. 1.

Fig. 1.

BRCA2 protein lacking exon11 is expressed in [Brca2F11/F11; Lck-Cre] mice. (A) Brca2 protein expression was detected in various mouse tissues. Each tissue was freshly isolated from 3-week-old mice, and 120 μg of protein was separated by SDS-PAGE and subjected to Western blot analysis. Lamin A/C was used as the loading control. (B) Western blot analysis of Brca2 was performed with thymic lysates from WT, [Brca2F11/+; Lck-Cre], and [Brca2F11/F11; Lck-Cre] mice. WT mice express the 400-kDa full-length Brca2 protein and [Brca2F11/F11; Lck-Cre] mice express the ∼220-kDa Brca2 fragment. (C) To analyse the Brca2 fragment from the [Brca2F11/F11; Lck-Cre] mice, RT-PCR was performed on thymic T cells. Sequence analysis of the 1.3-kb PCR product demonstrated joining of exons 10 and 12.