Fig. 3.

Loss of naïve T cells in [Brca2^F11/F11; Lck-Cre] mice. Splenic T cells were isolated from 4–8-week-old or 36–42-week-old WT, [Brca2^F11/+; Lck-Cre], or [Brca2^F11/F11; Lck-Cre] mice. (A) Cells were stained with FITC-ati-B220 and PE-anti-CD3 antibodies. Decreased T cell and increased B cell proportions are shown in the representative plots. Overall number of B cells remained unaltered. (25.9 × 10⁶ ± 8.2; 20 × 10⁶ ± 6.5; 21.7 ± 8 for WT; heterozygous; and homozygous mice respectively) (B) Cells were stained with [PerCP-anti-CD4, APC-anti-CD8, FITC-anti-CD44, and PE-anti-CD62L] antibodies. CD4⁺ or CD8⁺ T cells were gated and percentages of cells with CD44^low CD62L^high expression were calculated from the flow cytometry data. Representative contour plots for activation marker expression (CD44 vs.CD62L) on the gated CD4⁺ or CD8⁺ cells are shown. (C) Data were shown as average +/− SD from 3–8 mice for each genotype. P values < 0.05 from t-tests with the WT are marked as asterisks in the figure.