Fig. 4.
T cells from [Brca2F11/F11;Lck-Cre] mice show reduced immune responses. (A) CD4+ T cells were isolated from WT or [Brca2F11/F11; Lck-Cre] mice and stimulated with plate-bound anti-CD3 and anti-CD28 antibodies. Culture supernatants were collected after 48 h and subjected to cytokine ELISA. Experiments were repeated twice. The p values < 0.05 are marked as asterisks. (B) Sera were collected from WT and [Brca2F11/F11; Lck-Cre] mice 4 weeks after 2nd immunization with chicken ovalbumin, and the level of ovalbumin-specific antibodies were determined. Experiments were repeated twice and data points show average +/− SEM from 6 mice. Statistical significance was determined at dilution points giving ∼50% maximum O.D. readings, and the p-values < 0.05 are marked with asterisks for IgM and IgG1.