Fig. 2.
(A) Dose-dependent action of extracted SPM on hBMSC ALP activity at day 3. (B) Dose-dependent action of extracted SPM on hBMSC number at day 28. (C) Comparison of ALP activity at 24 h between SPM-treated (180 μg/ml) immunoselected hMSC and hMSCs cultured in osteogenic media (p < 0.001, n = 4). (D) Comparison of ALP activity at 21 days between SPM-treated (180 μg/ml) immuneselected hMSC and hMSCs cultured in osteogenic media (p < 0.01, n = 4). (E) Quantitative collagen binding assay comparing immouno-selected hMSCs treated with SPM and hMSCs without added SPM (p < 0.001, n = 4).