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. 2015 Mar 21;21(11):3239–3244. doi: 10.3748/wjg.v21.i11.3239

Figure 2.

Figure 2

Time-dependent expression of tumor necrosis factor-α in the early stage of lipopolysaccharide/D-galactosamine challenge in mice with or without urantide pretreatment. A: Representative ethidium bromide-stained gel of reverse transcription-PCR products from liver (M: DNA marker; Lines 1, 2, 3, 4, and 5: 0.0, 0.5, 1.0, 2.0, and 6.0 h after LPS/D-GalN challenge, respectively); B: Relative expression levels of TNF-α mRNA in the liver (normalized to β-actin); C: Levels of TNF-α secretion in blood as assayed by ELISA. Values are mean ± standard deviation (n = 6); aP < 0.05 vs 0 h; cP < 0.05 vs 6 h; eP < 0.05 vs mice without urantide pretreatment. TNF: Tumor necrosis factor; LPS: Lipopolysaccharide; D-GalN: D-galactosamine.