Table 2.
Primer sets used for real-time polymerase chain reaction
| Gene | Forward primer | Reverse primer |
| 5’ 3’ | 5’ 3’ | |
| Ferritin | CAGGTGCGCCAGAACTACCA | CCACATCATCGCGGTCAAAG |
| Transferrin | CGAAGACTGCATCGCCAAGA | ACACTTGCCCGCTATGTAGACAAAC |
| Hepcidin | AGCCTGACCAGTGGCTCTGT | TTCGCCTCTGGAACATGG |
| Ferroportin | AAGGGCAAGAATCCCAATTTAACTC | TGCCAGGCTGAAGGCTTACAC |
| TFR1 | GCATGTGGCATGTTCATCGTATAA | TCTCAAGACCAGGAGCTTGTCACTA |
| TFR2 | GCGACTGACACGCATGTACAAC | CCATGAAGATGTGGCGGAAC |
| DMT1 | CTTGCGAGGCAATCTCAGGA | CTGAGACAGTGAACTTTGCAACCA |
| IRP1 | GAAACAGTCCTGCTGCTCGCTAC | GAGCCATAGGAGTTGAATTCTCGTG |
| IRP2 | TTTATCTCCAGGCAGTGGGATG | CTGCGTCTGATAAGGGTGCTGTA |
| BMPR1 | TGGGAGTTGCTGCATTGCTGACC | ATGTAGCGTTTGGTGCCCACCC |
| BMPR2 | GCCACAAATGTCCTGGATGGCA | GAGGGGCGCCACCGCTTAAG |
| BMP2 | TTGCGCCAGGTCCTTTGACCAG | ACCTGGGGAAGCAGCAACGCTA |
| Hemojuvelin | TGCCAGACGGCTGTGCAAGG | CGGGCATCCTCCAGTGCTGC |
| RBBP6 | GCGACCTGCAGATCACCAA | TGCCATCGCTGGTTCAGTTC |
TFR: Transferrin receptor; DMT: Divalent metal transporter; IRP: Iron-regulatory protein; BMP: Bone morphogenetic protein; BMPR: BMP receptor; RBBP: Retinoblastoma binding protein.