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. 2015 Mar 17;10(3):e0120102. doi: 10.1371/journal.pone.0120102

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© 2015 O’Connor et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Analysis of Nfix expression during murine B cell differentiation using the B-cell lineage microarray dataset (GSE11110)[41]. Error bars represent max and min Nfix expression values. (B) Quantitative RT-PCR analysis of Nfix expression in murine B cell populations fractionated by flow cytometry. Data are representative of three independent experiments, performed in duplicate. Error bars denote ±SD. (C) Analysis of NFIX expression in human B cell development from the human hematopoiesis microarray dataset (GSE24759) (21). Plots represent raw values, error bars represent max and min values. (D) Analysis of Nfix expression by qRT-PCR in murine stem and progenitor cells isolated from bone marrow. (E) Graph of percentage expression of CD43⁺, B220⁺, and B220^loCD43⁺ populations in BM cells from chimeras established with MigR1 or Nfix progenitors 8–10 wks previously. Error bars denote +/- SD of 2 independent experiments (n = 3). (F) Representative flow cytometric analysis, with gates and percentages showing B220^loCD43⁺ populations (early B cells) in GFP^- and GFP⁺ populations.