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. 2015 Mar 17;10(3):e0119869. doi: 10.1371/journal.pone.0119869

Fig 1. Construction of TALENs and claudin-2 gene knockout in MDCK II cells.

Fig 1

(A) TALEN binding sites in the claudin-2 gene. The left and right arms of TALEN targeting site are indicated in blue and the spacer region is indicated in red. The initiating codon within the spacer region is shaded. (B) Immunofluorescence analysis of claudin-2 and ZO-1 in MDCK II cells transfected with TALEN constructs for claudin-2 gene knockout. After transfection, cells were subcultured on filter inserts for 4 days before analysis. Claudin-2 staining was completely lost at cell-cell contacts in claudin-2 knockout cells. Scale bar = 10 μm.