Figure 2.

Lucigenin-enhanced chemiluminescence assay. Effect of Agelanthus dodoneifolius subfractions on the chemiluminescence response of ROS generated by PMA-treated neutrophils cells. Data are expressed as mean ± SD of three parallel experiments. ^*** P < 0.0001 indicates significance compared to control solutions (one-way ANOVA followed by Dunnett's posttest). NS: not stimulated cells. The IC₅₀ values were 0.4 ± 0.2^a and 0.3 ± 0.1^a  μg/mL, respectively, for the ethyl acetate and butanolic subfraction. The IC₅₀ value of gallic acid was 0.4 ± 0.1^a  μg/mL. The letter (a) means that the IC₅₀ values were statistically similar (unpaired t-test with Welch's correction).