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Effects of catalpol on the nuclear localization of DAF-16. The translocation of DAF-16 was visualized under fluorescence microscope using TJ356 strain which carries daf-16::gfp transgene. To induce heat-shock, worms were incubated at 36°C for 2 h. Worms were subjected to analyze GFP expression on the 4th day of adulthood.
