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Activation of PPARα and stimulation of OCTN2 gene expression by PPARα agonist WY-14,643 in human and porcine cells. Human HepG2 cells and porcine PK-15 cells were treated without or with 150 μM WY-14,643 for 24 h to study the effect on CPT1 mRNA level (A), OCTN2 mRNA level (B) and OCTN2 protein level (C). Representative immunoblots for OCTN2 and β-actin are shown. Bars represent means ± SD for three independent experiments. *Different from DMSO-treated control, P < 0.05.
