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Binding ofin vitrotranslated mouse PPARα/RXRα to the PPRE in the intron 1 of porcine, bovine and human OCTN2 gene. EMSA was performed using DIG-labeled oligonucleotides corresponding to the wild-type and the mutated PPRE of porcine (A), bovine (B), and human (C) OCTN2 and in vitro-translated mouse PPARα/RXRα. DIG-labeled specific and non-specific probes were used as positive and negative controls, respectively. Arrows indicate DNA/protein complexes and free DNA probes.
