Table 2.
Oligonucleotides used for cloning, mutagenesis and EMSA
| Denomination of oligonucleotides | Oligonucleotide sequences | PCR product size (bp) | Accession number |
|---|---|---|---|
| Oligos used for cloning | |||
| pOCTN2-PPRE-HindIII | ATAAAGCTTCAGCCTCTCTGTTTCGTCAG | 228 | CU372899 |
| pOCTN2-PPRE-XhoI | ATACTCGAGGAGCTATGTTGCTGCCAGTG | ||
| bOCTN2-PPRE-HindIII | ATAAAGCTTCAGAAGGGTCCTTGAGCTAT | 243 | AC149665 |
| bOCTN2-PPRE-XhoI | ATACTCGAGCCAACAGTGACTGTTCACCA | ||
| hOCTN2-PPRE-HindIII | ATAAAGCTTGCTCTGAACTTCAAGTCAAGC | 246 | AC000137 |
| hOCTN2-PPRE-XhoI | ATACTCGAGCTGAGTGATGGTGGCATTGA | ||
| Oligos used for mutagenesis and EMSA-mut | |||
| pOCTN2-PPREmut-For | AACCTGTAAGTAGGTGTATGGGCACACAACTCGTA | ||
| pOCTN2-PPREmut-Rev | TACGAGTTGTGTGCCCATACACCTACTTACAGGTT | ||
| bOCTN2-PPREmut-For | AACCTGGAAGTAGGTGTATGGGCACAGAGCTCTTT | ||
| bOCTN2-PPREmut-Rev | AAAGAGCTCTGTGCCCATACACCTACTTCCAGGTT | ||
| hOCTN2-PPREmut-For | AACATATAAGTAGGTGtAtGGGCACATAACTCCTT | ||
| hOCTN2-PPREmut-Rev | AAGGAGTTATGTGCCCaTaCACCTACTTATATGTT | ||
| Oligos used for EMSA | |||
| pOCTN2-PPRE-For | AACCTGTAAGTAGGTGAAAGGGCACACAACTCGTA | ||
| pOCTN2-PPRE-Rev | TACGAGTTGTGTGCCCTTTCACCTACTTACAGGTT | ||
| bOCTN2-PPRE-For | AACCTGGAAGTAGGTGAAAGGGCACAGAGCTCTTT | ||
| bOCTN2-PPRE-Rev | AAAGAGCTCTGTGCCCTTTCACCTACTTCCAGGTT | ||
| hOCTN2-PPRE-For | AACATATAAGTAGGTGAAAGGGCACATAACTCCTT | ||
| hOCTN2-PPRE-Rev | AAGGAGTTATGTGCCCTTTCACCTACTTATATGTT | ||
| EMSA-sp.-For | TCTTCCCGAACGTGACCTTTGTCCTGGTCCCCTTT | ||
| EMSA-sp.-Rev | TCAAAGGGGACCAGGACAAAGGTCACGTTCGGGAA | ||
| EMSA-non-sp.-For | TTCCCATCTTGTGAGCTGTCACCCATGGTGGGGTG | ||
| EMSA-non-sp.-Rev | CACCCCACCATGGGTGACAGCTCACAAGATGGGAA |
Abbreviations: b bovine, h human, p porcine.