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. 2015 Mar 2;2015:189785. doi: 10.1155/2015/189785

Figure 5.

Figure 5

The effect of NAC on autophagy in ConA-induced hepatitis. (a) The expressions of LC3 and Beclin 1 on mRNA levels were detected by real-time PCR. Data are expressed as mean ± SD (n = 6; * P < 0.05 for ConA versus NAC 30 min; # P < 0.05 for NAC 30 min versus NAC 2 h). (b) The expressions of LC3 and Beclin 1 on protein levels were detected with western blot. The expressions of LC3 and Beclin 1 on protein levels of macrophages and hepatocytes were detected by western blot at 12 h time point. The results of western blot were analyzed with Quantity One. Data are expressed as mean ± SD (n = 6; * P < 0.05 for ConA versus NAC 30 min; # P < 0.05 for NAC 30 min versus NAC 2 h). (c) The expressions of LC3 and Beclin 1 on protein levels were detected by immunohistochemistry staining in hepatic tissues at 12 h (×200 and ×400 magnification). The positive cells were indicated with red arrows. The result was analyzed using Image-Pro Plus 6.0. Date are showed as mean ± SD (n = 6; * P < 0.05 for ConA versus NAC 30 min; # P < 0.05 for NAC 30 min versus NAC 2 h). (d) Morphology of autophagosomes in hepatocytes at 12 h detected by electron microscopy (×20000 magnification).