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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Cell Res. 2014 Oct 16;330(1):199–211. doi: 10.1016/j.yexcr.2014.10.003

Fig. 3. ERK5 inhibition selectively increases the expression of M-CSFR, a molecular marker of macrophage differentiation, at mRNA level.

Fig. 3

HL60 or U937 cells were pretreated with either MEK1/2 inhibitors, PD98059 (20 µM) and U0126 (1 µM) or MEK5/ERK5 inhibitors, BIX02189 (10 µM) and XMD8-92 (5 µM), for 1 h, then 1,25D (1 nM) was added for an additional 96 h. The levels of M-CSFR mRNA were determined by SYBRGreen RT-qPCR in both HL60 and U937 cells. ◆ = p<0.05, ◆◆ = p<0.01, significantly increased vs. control group; ◇ = p<0.05, significantly decreased vs. control group; # = p<0.05, ## = p<0.01, significantly increased vs. 1,25D-treated group.