. Author manuscript; available in PMC: 2016 Jan 1.

Published in final edited form as: Exp Cell Res. 2014 Oct 16;330(1):199–211. doi: 10.1016/j.yexcr.2014.10.003

Table 1. Quantitation of phagocytic activity following induction of macrophage phenotype.

Following treatments and phagocytosis assay (illustrated in Fig. 6, A and B) at least 500 cells were counted on each slide. The percentage of cells containing two or more phagocytized particles of opsonized zymosan was calculated and expressed as the mean ± S.D. (n=3).

		Level of significance
	Phagocytosis (%)	vs. Control	vs. 1,25D alone	vs. Inhibitor alone
HL60 cells
Control	0.2 ± 0.4
D₃-1 nM	6.1 ± 2.2	^**
PD-20 µM	0.9 ± 0.1	^*
D₃+PD	2.8 ± 0.6	^**	ns	^**
U0126-1 µM	0.6 ± 0.6	ns
D₃+U0126	4.9 ± 2.2	^*	ns	^*
BIX-10 µM	1.7 ± 0.8	^*
D₃+BIX	10.2 ± 1.4	^***	ns	^***
XMD-5 µM	6.2 ± 1.4	^**
D₃+XMD	48.0 ± 3.8	^****	^****	^****
TPA 10 nM	22.0 ± 4.9	^**
U937 cells
Control	0.8 ± 1.0
D₃-1 nM	6.0 ± 1.1	^**
PD-20 µM	2.6 ± 1.0	ns
D₃+PD	6.8 ± 2.8	^*	ns	ns
U0126-1 µM	1.0 ± 0.8	ns
D₃+U0126	6.5 ± 2.8	^*	ns	^**
BIX-10 µM	1.4 ± 0.6	ns
D₃+BIX	9.2 ± 1.8	^***	^*	^***
XMD-5 µM	4.4 ± 0.7	^*
D₃+XMD	22.0 ± 4.8	^***	^***	^**
TPA 10 nM	14.5 ± 3.5	^***

= p<0.05;

^**

= p<0.01;

^***

= p<0.001;

^****

= p<0.0001.