Fig. 2.

Expression of cPLA₂α (a), sPLA₂-V (b) and iPLA₂c in WT and iPLA₂β^−/− mice. mRNA was quantified in duplicate with Taqman® RT-PCR using the ΔΔCt method and normalization to 18S expression. Results are expressed as mean ± SEM (n = 8). cPLA₂α and sPLA_2-V protein was measured in brain cytosol and P-cPLA₂α was quantified in the membrane fraction by Western blot. Results are normalized to β-actin expression (representative immunoblot is displayed) and expressed as % of the WT mice as mean ± SEM (n = 8, except n = 7 for P-cPLA₂ in iPLA₂β^−/− because an outlier value of 7.4 was removed). Numbers in italics indicate molecular weight markers (in kDa), NS : non-specific band. *P < 0.05 (t-test)