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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Neurochem Res. 2014 Jun 12;39(8):1522–1532. doi: 10.1007/s11064-014-1342-y

Fig. 6.

Fig. 6

Expression of microglial, astrocytic, synaptic markers and BNDF in WT and iPLA2β−/− mice. mRNA was quantified in duplicate with Taqman® RT-PCR using the ΔΔCt method and normalization to 18S expression. Results are expressed as mean ± SEM (n = 8). CD11b, synaptophysin and PSD-95 expression was measured in brain membrane fractions by Western blot and GFAP, drebrin and BDNF were quantified in the cytosol. Results are normalized to β-actin expression (representative immunoblot is displayed) and expressed as % of the WT mice as mean ± SEM (n = 8 except n = 7 for BDNF in WT and iPLA2β−/− , respectively due to the removal of an outlier value of 222 and sample loss). Numbers in italics indicate molecular weight markers (in kDa). *P < 0.05, **P < 0.01 (t test) and #0.5 < d > 0.8 (Cohen's d test)