Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 17;10(3):e0118865. doi: 10.1371/journal.pone.0118865

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Merrick et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 2 — A: Quantitative RT-PCR analysis of PfSir2a and PfSir2b expression in schizont-stage 3D7, NF54 and FCR-3. RCN is calculated by comparison to the average abundance of transcripts encoding two control genes: seryl-tRNA-synthetase and myosin. Plot shows average of 2 biological replicates, measured in technical duplicate. B: Table showing sirtuin gene disruptions attempted and achieved in 3D7, NF54 and FCR-3. C: Southern blots showing disruption of PfSir2b in NF54, PfSir2a and PfSir2b in FCR-3, and attempted but unsuccessful disruption of PfSir2a in NF54. The expected sizes for the FCR-3Δsir2a and NF54Δsir2a endogenous genomic locus, integrated flank 1, integrated flank 2 and concatamerized plasmid following AccI/NdeI digestion are 1.1 kb, 3.9 kb, 2.4 kb and 5.2 kb, respectively. The expected sizes for the FCR-3Δsir2b and NF54Δsir2b endogenous genomic locus, integrated flank 1, integrated flank 2 and concatamerized plasmid following ScaI/HpaII digestion are 1.7 kb, 4.2 kb, 2.1 kb and 4.5 kb. D: Var expression patterns in two clones each of NF54 wt, NF54Δsir2b, FCR-3 wt, FCR-3Δsir2a and FCR-3Δsir2b measured by qRT-PCR using gene-specific primers for the majority of the var gene family, sorted by ups-group. RCN was calculated by comparison to the abundance of transcripts encoding the average of the three control genes seryl-tRNA synthetase, arginyl-tRNA synthetase and glutaminyl-tRNA synthetase. Individual clones denoted in black and brown bars. Pie charts define percentage of each ups class expressed, and are sized proportional to the total RCN. ns = no significant difference between total var gene expression levels in the individual strains.