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. 2015 Mar 17;10(3):e0120642. doi: 10.1371/journal.pone.0120642

Fig 4. Flow cytometry gating strategy for ascites leukocyte characterisation.

Fig 4

(A) Lymphocytes and myeloid cells were distinguished on the basis of side scatter properties and CD14 expression. CD14Hi and CD14 Low/negative cells were further characterised for CD16, HLA-DR and CD66B expression (top right panels). Lymphoid cells were classified as B cells (CD19+), T cells (CD3+), and NK cells (CD56+/CD16+/-) (bottom left panels). (B) Myeloid populations were further investigated for surface CD11C, CCR2, CD163 and CX3CR1 expression.