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. 2015 Mar 17;10(3):e0120628. doi: 10.1371/journal.pone.0120628

Fig 1. Nkx3.1-loss modestly increases the Tmprss2 promoter activity in vivo.

Fig 1

A. Progressive Nkx3.1 transcript loss was confirmed in wild type (black) and heterozygous (dark gray) and homozygous (light gray) Nkx3.1 knockout mice by real-time RT-PCR (left). Immunohistochemical (IHC) staining of anterior prostates (APs) using a mouse-specific Nkx3.1 antibody also validated Nkx3.1 protein loss. B. Real-time RT-PCR showing slight but statistically significant increase in the Tmprss2-ERG expression in T-ERG;Nkx3.1 +/- double heterozygous males. C. IHC staining of APs showing increase in ectopic ERG expression at the protein level from the T-ERG knockin allele under the Nkx3.1-null background (T-ERG;Nkx3.1 -/-). H-scores were calculated as 81 and 127 for T-ERG and T-ERG;Nkx3.1 -/- sections, respectively. D. FACS analysis showing progressive increase in the percentage of GFP+ cells in the prostates of T-ERG;Nkx3.1 +/- and T-ERG;Nkx3.1 -/- males, compared to those of males with T-ERG alone. Statistics: p values from Student t-test are indicated; ns = not significant. Scale bars represent 50 μm.