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. 2015 Mar 17;10(3):e0121468. doi: 10.1371/journal.pone.0121468

Fig 3. Identification of a CRE/AP-1-like motif and GATA-binding site within the Syncytin-2 promoter.

Fig 3

(A) Extended probe 5 (termed WT) (from-211 to-177) (indicated in the upper part of the panel) was used for EMSA analyses and highlights the CRE/AP-1-like motif and the potential GATA-binding site in the Syncytin-2 promoter. Nuclear extracts from BeWo cells treated (T) or not (NT) with forskolin for 15 min were pre-incubated with different concentrations of cold specific or non-specific oligonucleotides prior to addition of the labeled WT probe. (B) EMSA analyses of nuclear extracts from BeWo cells stimulated with forskolin at different time points and incubated with the WT probe. (C) Representation of the various oligonucleotides bearing mutated sequences (nucleotides in italic) used in EMSA analyses. (D-E) Nuclear extracts from non-treated (NT) or forskolin-treated (T) BeWo cells were incubated with the WT probe and excess (100X) cold WT or mutated oligonucleotides presented in (C). Specific DNA-protein complexes are indicated on left side of panels. (F) Nuclear extracts from primary villous cytotrophoblasts cultured for 24 and 96 hours were incubated with the WT probe and with or without excess (100X) cold WT oligonucleotide.