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. 2014 Jul 28;14:67. doi: 10.1186/1475-2867-14-67

Figure 4.

Figure 4

Effect of RelB expression on cell proliferation and anchorage-independent cell growth of 22Rv1 cells. A) Representative field of 22Rv1 colonies (macroscopic view) illustrating 22Rv1 RelB and GFP cells grown in soft agar. B) Soft agar growth assay. 22Rv1 cells were seeded in soft agar and incubated for 2 weeks. Colonies were stained with crystal violet. Graph represented the number of colonies in soft agar. Experiments were done three times in duplicate. Error bars represent the standard error of the mean and p < 0.05 was considered a significant variation (Mann- Whitney U test). C) Growth curves by cell counting. 22Rv1 RelB clones and GFP control cells were seeded at day 0. The first cell count was done at day 1 and after, cells were counted every 2 days until day 9 (10 days after cell seeding). The relative cell growth was obtained by normalizing data to the cell count at day 1. Experiments were done three times in duplicate. Error bars on the graph represent the standard error of the mean and we consider a p < 0.05 significant variation (Anova one-way followed by Tukey post-test).