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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Microbiol. 2014 Dec 19;95(2):270–282. doi: 10.1111/mmi.12863

Figure 1.

Figure 1

Mapping of 5′ ends of tmRNA precursors. (A) Primer extension assay mapping the 5′ ends of transcripts from the putative P1 and P2 promoters and the 5′ end of the mature tmRNA (oligo CC1444). Reactions were performed on RNA isolated from wild-type (wt) and RNase P-depleted (P↓) strains, with the latter strain showing increased accumulation of P1 and P2-originating transcripts. Sequence lanes are labelled as their reverse complement to facilitate direct read-out. The origin of the species marked with an asterisk migrating just above the mature tmRNA is unknown. (B) Sequence of the ssrA gene, encoding tmRNA. The −35 and −10 regions of the putative P1 and P2 promoters are highlighted in grey and the 5′ ends of the corresponding transcripts labelled as +1 (underlined). The tmRNA is indicated in bold type, with the confirmed 5′ end of the mature sequence indicated by M. Mapped 5′ ends of degradation intermediates (DI) identified in cells depleted for RNase Z are indicated. The transcription terminator is underlined.