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AQP1 blockade in H226 cells results in reduced colony number and size in an anchorage independent assay. (a) Number of colonies: n = 3, P = 0.001. Analysed by one-way ANOVA followed by Tukey's post hoc analysis (A, B, and C indicate significant subgroups, P ≤ 0.05). (b) Size of cell colonies: n = 3, P = 0.039. Analysed by independent samples t-test. Significant differences indicated by ∗. Error bars: SD. V: DMSO vehicle control.
