Figure 2.

Surface molecules of DCs loaded with DRibbles.

Notes: DCs were incubated with DRibbles (5, 25, and 50 μg/mL) for 12 h. MHC-I, MHC-II, the costimulatory molecules of CD40, CD86 were analyzed by flow cytometry. CD11c was used as the maker to gate DCs. (A) CD40 expression on DCs loaded with different concentrations of DRibbles; (B) CD86 expression on DCs loaded with different concentrations of DRibbles; (C) MHC-II expression on DCs loaded with different concentrations of DRibbles; (D) MHC-I expression on DCs loaded with different concentrations of DRibbles.

Abbreviations: DCs, dendritic cells; DRibbles, tumor-derived autophagosomes; MHC, major histocompatibility complex.