Figure 4.

Apoptosis and function of DCs loaded with DRibbles.

Notes: (A) Apoptosis rate of DCs (1×10⁶) loaded with either DRibbles (25 μg/mL) or tumor lysate (25 μg/mL) for 12 hours were performed by flow cytometry. DCs were stained with annexin V-FITC and PI; (B) DCs (2×10⁴) loaded with either DRibbles (25 μg/mL) or lysate (25 μg/mL) for 12 hours with or without cytokine cocktails, such as TNFα (10 ng/mL), IL-1β (10 ng/mL), IL-6 (10 ng/mL), and PGE₂ (1 μg/mL) were incubated with T cells (2×10⁵) obtained from either lymph nodes or spleen. T cells were purified by magnetic bead separation. After being incubated for 72 hours, the activation and proliferation of T cells were assayed using Cell Counting Kit 8, and the absorbances were read at 450 nm. *P<0.05; **P<0.01; ***P<0.001.

Abbreviations: DCs, dendritic cells; DRibbles, tumor-derived autophagosomes; FITC, fluorescein isothiocyanate; PI, propidium iodide; PG, prostaglandin; LN-MLR, mixed lymphocyte reaction and T cells derived from lymph nodes in the reaction; P-MLR, mixed lymphocyte reaction and T cells derived from spleen in the reaction.