Figure 5.

Booster vaccination with DRibble-loaded DCs delayed the tumor.

Notes: C3H/HeJ mice (n=24, 8 mice each group) carcinoma models were established by receiving subcutaneous (SC) injection of 2×10⁶ SCC7 cells in the right flank on day 0. These carcinoma models were given 1×10⁶ DCs loaded with either DRibbles (25 μg/mL) or tumor lysate (25 μg/mL) via SC injection in footpads on day 7 and intranodal (IN) injection on day 14. Meanwhile, those treated with PBS served as controls. During the whole observation, the tumor volume of tumor-bearing mice was monitored every 3 days. (A) Vaccine scheme in an established murine carcinoma model; (B) percentage of survival; (C) tumor volume.

Abbreviations: DRibble, tumor-derived autophagosome; DCs, dendritic cells; PBS, phosphate-buffered saline.