Fig. 3.

Transcriptional profiling of PBMCs is sensitive and reveals enrichment of effector T-cell genes. (A) We performed transcriptional profiling of PBMCs obtained from vaccinees before and days 3 and 7 after vaccination. Genes whose absolute level of expression correlates positively (red) or negatively (blue) with viral load on the day of PBMC sampling are shown as a heat map. The viral load corresponding to each transcriptional profile is shown as a bar graph above the heat map. (B) The transcriptional profiles of YFV-specific effector CD8 T cells or prevaccination naive CD8 T cells were tested for enrichment among the genes that were associated with viral load from A. The GSEA plots are shown. (C) Expression profiles of various published immune cell subsets were tested for enrichment among the viral load associated genes. Normalized enrichment score for most significantly associated gene sets is shown.