Fig. 5.

The cipk3/9/23/26 quadruple mutant was hypersensitive to high-Mg stress. (A–C) Phenotype analysis of Col-0, cbl2 cbl3, and cipk3/9/23/26 seedlings under high-Mg conditions. Four-day-old seedlings grown on MS medium were transferred onto MS medium (A) or MS medium supplemented with 10 mM (B) or 20 mM (C) MgCl₂. Photographs were taken on the 14th day after the transfer. (D) Fresh weight of the seedlings on the 14th day after the transfer. Data shown are the mean ± SE of four replicate experiments. (E and F) Mg (E) and Ca (F) content of Col-0 and cipk3/9/23/26 seedlings under different Mg²⁺ regimes. Data are presented as the mean ± SE of triplicate experiments. Asterisks indicate statistically significant differences compared with wild-type Col-0 (Student’s t test, *P < 0.05).