Fig. 5.

Negative selection against maintaining Reaper expression during mosquito infection by MRE/Rpr. (A) Strategy used for Sanger sequencing. Viruses collected from infected mosquitoes were isolated by plaque assay, and the region of the viral genome containing the duplicated subgenomic promoter and insert was amplified and sequenced from 8 to 10 plaque isolates from each of 10 mosquitoes. (B) Each data point indicates the percentage of sampled viruses (n = 8–10 plaque isolates) containing intact inserts from a single mosquito. The results are also presented in Table S1. Data obtained from sequencing individual plaque isolates obtained from stock viruses are indicated by red shapes (n = 25–40 plaque isolates). Modified with permission from ref. 60.