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. 2015 Mar 2;112(10):3092–3097. doi: 10.1073/pnas.1419388112

Fig. 3.

Fig. 3.

FlhG interacts with the flagellar C-ring proteins FliM/FliY. (A) Coomassie-stained SDS/PAGE of an in vitro pulldown assay of GST-GtFlhG and GtFliM/FliY in the absence and presence of ADP, ATP, or AMPPNP. (B) Deuterium incorporation of depicted peptides of free protein and the dimeric GtFlhG/FliY complex are given in percent H/D exchange. Decreased deuterium content upon complex formation indicates potential interfaces (peptides GtFliY R1: DALLRGMDDSDHVPALH; GtFlhG P1: TDAYAMMKYMHAAGSEAPFSV and P2: VFERLKHVTGRFLNKD). (C) Coomassie-stained SDS/PAGE of an in vitro pulldown assay using (His)6-tagged GtFlhG, GtFliM/FliY, and GtFliM/FliY variants lacking the FliY-Ntr and FliM-Ntr. (D) Coomassie-stained SDS/PAGE of an in vitro pulldown assay using different (His)6-tagged GtFlhG variants and the GtFliM/GtFliY complex. (E) Major differences that allow FlhG (Left) and MinD (Right) to bind FliM/FliY and MinC, respectively, are shown in blue.