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. 2015 Mar 2;112(10):3092–3097. doi: 10.1073/pnas.1419388112

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Fig. 4. — Physiological role of FlhG. (A) Localization of a BsFlhG-YFP fusion protein in B. subtilis displays distinct foci at the membrane. (B and C) Localization of FlhG and FliM was detected in B. subtilis carrying BsFlhG-YFP (green) and BsFliM-CFP (red). (D) Coomassie-stained SDS/PAGE of an in vitro pulldown assay of GST-GtFliG with GtFliM/FliY in the absence and presence of GtFlhG. (E) Coomassie-stained SDS/PAGE investigating the ability of GtFlhG to bind to GST-GtFliG and its dependence on lipids, ADP, ADP+lipids, ATP, and ATP+lipids. (F) Coomassie-stained SDS/PAGE of a time-resolved pulldown assay (0, 1, 5, 10, and 30 min) investigating the binding of GtFliM/FliY and GtFlhG to GST-GtFliG in presence of ATP and lipids.