Fig. 1.

Reporters for studying termination readthrough at PTCs. (A) Schematic representation of the HA-LUC_(PTC20)-SF reporter with an in-frame N-terminal HA tag, followed by the luciferase ORF and C-terminal SF tags. Nucleotide substitutions encoding PTCs (UAA, UAG, or UGA) were inserted at the position of amino acid 20 of luciferase. (B) Northern blot analysis of the HA-LUC-SF mRNA expressed in either WT [PSI⁻] (Left) or upf1∆ [PSI⁻] (Right) cells. Blots were reprobed for CYH2 transcripts as an internal NMD control. SCR1 RNA was used as a loading control.