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Amino acids inserted at PTCs during termination readthrough. HA-LUC(PTC20)-SF protein products purified from WT [PSI−] cells, upfΔ [PSI−] mutants, termination factor mutants, or WT [PSI−] cells treated with gentamicin (WT + gent) were subjected to MS analyses. The numbers denote the frequency (±SD of the mean) of insertion of the amino acids at UAA (A), UAG (B), or UGA (C) (n = 3). The type and position of mispairing in the codon are represented for each amino acid inserted.
