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. 2015 Feb 23;112(10):E1106–E1115. doi: 10.1073/pnas.1420955112

Fig. 1.

Fig. 1.

Flow diagram depicting the steps taken in identifying novel miRNAs. Shown is a flow diagram of the process to identify candidate novel miRNAs from 1,323 deep-sequencing samples using miRDeep2. Only mature miRNA with associated FDR ≤ 0.05 were kept for further analysis. Discovered sequences that were present in release 20 of miRBase, or overlapped known tRNAs, snRNAs, or rRNAs were discarded. A total of 3,707 candidate miRNAs derived from 3,494 precursor sequences were identified. Intersection of the identified miRNAs with 43 Ago-CLIP-seq samples showed evidence of Ago loading for 1,657 newly discovered miRNAs. Sixty-six of the identified precursors produced two miRNAs, one from each arm: one product was supported by Ago CLIP-seq whereas the other was not.