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. 2015 Feb 23;112(10):3002–3007. doi: 10.1073/pnas.1420024112

Fig. 2.

Fig. 2.

Application of multicolor CRISPR to telomere labeling in live cells. (A) Sp dCas9-3xCherry (Middle Left) and Nm dCas9-3xGFP (Middle Right) were coexpressed in U2OS cells together with their cognate sgRNAs (Sp sgRNA-Telo and Nm sgRNA-Telo) targeting to telomeres. Shown are phase-contrast images (Left) and the merged fluorescence image (Right). (B) Sp dCas9-3xGFP (Middle Left) and St1 dCas9-3XBFP (Middle Right) were coexpressed in U2OS cells together with their cognate sgRNAs (Sp sgRNA-Telo and St1 sgRNA-Telo) targeting to telomeres. Shown are phase-contrast images (Left) and the merged fluorescence image (Right). (Scale bar: 5 μm.)