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. 2015 Feb 23;112(10):3002–3007. doi: 10.1073/pnas.1420024112

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Fig. 3. — Interchromosomal and intrachromosomal loci labeling by multicolor CRISPR. Pericentromeric satellite DNA in human chromosomes 9 (C9-1) and repeat sequences unique to chromosome 9 (C9-2) or chromosome 13 (C13-1) are shown at the top. (A) Plasmids encoding Sp dCas9-3XCherry or St1 dCas9-3XGFP were cotransfected into RPE-1 cells with cognate sgRNAs (Sp sgRNA C9-1 or St1 sgRNA C9-1) targeting to pericentromeric satellite DNA in human chromosome 9. Phase-contrast images (Left and Middle Right) and fluorescent images (Middle Left and Right) are shown. (B and C) St1 dCas9-3xGFP and Sp dCas9-3XCherry were coexpressed in RPE-1 cells with their cognate sgRNAs (St1 sgRNA C9-1 and Sp sgRNA C13-1) (B) or in U2OS cells with cognate sgRNAs (St1 sgRNA C9-1 and Sp sgRNA C9-2) (C). Shown are phase-contrast (Left in B and C), GFP (Middle Left in B and C), Cherry (Middle Right in B and C), and the merged fluorescence (Right in B and C) images. (Scale bar: 5 μm.)