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. 2015 Mar 2;112(10):3080–3085. doi: 10.1073/pnas.1419699112

Fig. 3.

Fig. 3.

Transcriptional induction of Il6 and Il1rl1 by Hes1. (A) Immunofluorescence from antibodies to Il6 or Il1rl1 in mouse knee joints 4 wk after creating a surgical OA model in 8-wk-old Hes1fl/fl (Cntl) or Col2a1-CreERT;Hes1fl/fl (cKO) littermates. (Scale bars, 100 μm.) (B) Luciferase activities following transfection of GFP or HES1 into HeLa or SW1353 cells with a reporter construct containing a wild-type (WT) fragment from IL6 or a fragment with a mutation in the E-box (mut), as indicated (Upper). Data are shown as means ± SD of three wells per group. *P < 0.05 versus HES1 in WT. (C) Luciferase activities following the transfections of GFP or HES1 into HeLa or SW1353 cells with a reporter construct containing a wild-type (WT) fragment from IL1RL1 or a fragment with a mutation in N1, E1, E2&3, E4, or E5&6, as indicated in (Upper). Data are shown as means ± SD of three wells per group. *P < 0.05 vs. HES1 in WT.