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. 2014 Dec 22;13:200. doi: 10.1186/1476-511X-13-200

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© de Almeida et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Analysis of PPARγ protein level in retroperitoneal adipose tissue. PPARγ levels (A) and representative blot for PPARγ and β-tubulin (loading control) (additional Electrophoretic blot files show this in more detail [see Additional files 1, 2, 3 and 4]) (B) of male Wistar rats fed the following dietary treatments for 60 days: Normal fat-Soybean oil (NF-So): diet containing 4.0% soybean oil (SO); High Fat-Control Butter (HF-Cb): diet containing 21.7% control butter and 2.3% SO; High Fat-CLA enriched Butter (HF-CLAb): diet containing 21.7% cis-9, trans-11 CLA-enriched butter and 2.3% SO; High fat-Soybean oil (HF-So): diet containing 24.0% SO. All data are presented as mean values ± S.E.M (n = 10 rats/group). Statistically significant differences were determined by Anova followed by Newman-Keuls. *p <0.05, **p <0.01.