Table 3.
Chemical composition and fatty acid profile of the experimental diets
| Dietary treatments | ||||
|---|---|---|---|---|
| NF-So2 | HF-Cb3 | HF-CLAb4 | HF-So5 | |
| Chemical composition,% of diet dry matter | ||||
| Dry matter content (%) | 79.1 | 86.8 | 85.4 | 88.4 |
| Fat | 3.11 | 17.6 | 17.4 | 21.1 |
| Crude protein | 13.1 | 16.0 | 16.2 | 14.8 |
| Ash | 2.76 | 2.98 | 3.09 | 2.95 |
| Neutral Detergent Fiber (NDF) | 2.76 | 3.55 | 3.26 | 3.89 |
| Carbohydrate | 55.4 | 44.8 | 43.4 | 42.7 |
| Energetic composition | ||||
| Carbohydrate Energy (%) | 73.4 | 44.6 | 43.9 | 40.7 |
| Protein Energy (%) | 17.4 | 15.9 | 16.4 | 14.1 |
| Fat Energy (%) | 9.35 | 39.5 | 39.6 | 45.2 |
| Kcal/g | 2.39 | 3.49 | 3.38 | 3.71 |
| Fatty acids (g/100 g of total fatty acids) | ||||
| C4:0 | n.d.1 | 3.16 | 2.95 | n.d. |
| C5:0 | n.d. | 0.03 | 0.01 | n.d. |
| C6:0 | n.d. | 1.69 | 1.37 | n.d. |
| C7:0 | n.d. | 0.02 | 0.01 | n.d. |
| C8:0 | n.d. | 1.00 | 0.64 | n.d. |
| C9:0 | n.d. | 0.03 | 0.01 | n.d. |
| C10:0 | n.d. | 2.07 | 1.14 | n.d. |
| C10:1 cis -9 | n.d. | 0.26 | 0.12 | n.d. |
| C11:0 | n.d. | 0.02 | 0.01 | n.d. |
| C12:0 | n.d. | 2.37 | 1.25 | n.d. |
| C12:1 cis -9/C13:0 | n.d. | 0.16 | 0.08 | n.d. |
| C14:0 | 0.52 | 8.71 | 5.74 | 0.54 |
| C15:0 iso | n.d. | 0.20 | 0.25 | n.d. |
| C15:0 anteiso | n.d. | 0.41 | 0.47 | n.d. |
| C14:1 cis -9 | n.d. | 0.83 | 0.46 | n.d. |
| C15:0 | n.d. | 0.95 | 0.90 | n.d. |
| C16:0 | 11.7 | 29.3 | 19.7 | 11.8 |
| C16:1 trans -9 | n.d. | 0.03 | 0.03 | n.d. |
| C17:0 iso | n.d. | 0.32 | 0.51 | n.d. |
| C16:1 cis -9 + C17:0 anteiso | n.d. | 1.51 | 1.16 | n.d. |
| C17:0 | n.d. | 0.49 | 0.51 | n.d. |
| C17:1 cis -9 | n.d. | 0.18 | 0.19 | n.d. |
| C18:0 | 4.25 | 9.02 | 13.9 | 4.23 |
| C18:1 trans -4 | n.d. | 0.02 | 0.07 | n.d. |
| C18:1 trans -5 | n.d. | 0.02 | 0.06 | n.d. |
| C18:1 trans -6/7/8 | n.d. | 0.31 | 0.80 | n.d. |
| C18:1 trans -9 | n.d. | 0.23 | 0.57 | n.d. |
| C18:1 trans -10 | n.d. | 0.30 | 0.81 | n.d. |
| C18:1 trans -11 | n.d. | 1.09 | 4.03 | n.d. |
| C18:1 trans -12 | n.d. | 0.29 | 0.65 | n.d. |
| C18:1 trans -13/14 | n.d. | 0.24 | 0.49 | n.d. |
| C18:1 cis- 9/ trans -15 | 23.8 | 20.3 | 25.9 | 22.4 |
| Minor cis -C18:1 isomers (c11 + c12 + c13) | 1.43 | 0.83 | 1.03 | 1.45 |
| C18:1 trans -16 | n.d. | 0.23 | 0.36 | n.d. |
| C18:1 cis -14 | n.d. | 0.05 | 0.10 | n.d. |
| C19:0/C18:1 cis -15 | n.d. | 0.11 | 0.11 | n.d. |
| C18:2 trans -9 trans -12 | n.d. | 0.01 | 0.01 | n.d. |
| C18:2 cis -9 trans -12 | 0.09 | 0.04 | 0.06 | 0.08 |
| C18:2 trans -9 cis -12 | n.d. | 0.03 | 0.04 | n.d. |
| C18:2 cis -9 cis -12 | 49.5 | 8.04 | 7.15 | 52.4 |
| C20:0 | 0.36 | 0.18 | 0.20 | 0.35 |
| C18:3 cis -6, cis -9 cis -12 | n.d. | 0.02 | 0.01 | n.d. |
| C20:1 cis -11 | n.d. | 0.06 | 0.12 | n.d. |
| C18:3 cis -9 cis -12 cis -15 | 6.16 | 0.96 | 0.89 | 6.58 |
| CLA cis -9 trans -11 | n.d. | 0.53 | 1.66 | n.d. |
| CLA trans -10 cis -12 | n.d. | 0.01 | 0.01 | n.d. |
| CLA trans -11 cis -13 | n.d. | 0.01 | 0.02 | n.d. |
| C21:0 | n.d. | 0.03 | 0.03 | n.d. |
| C20:2 cis -11, cis -14 | n.d. | 0.02 | 0.02 | n.d. |
| C22:0 | 0.41 | 0.11 | 0.13 | 0.30 |
| C20:3 n-6 | n.d. | 0.05 | 0.04 | n.d. |
| C20:4 n-6 | n.d. | 0.10 | 0.08 | n.d. |
| C23:0 | n.d. | 0.03 | 0.01 | n.d. |
| C20:5 n-3 (EPA) | n.d. | 0.02 | 0.01 | n.d. |
| C24:0 | 0.15 | 0.06 | 0.06 | 0.16 |
| C22:5 n3 (DPA) | n.d. | 0.06 | 0.06 | n.d. |
| C22:6 n-3 (DHA) | n.d. | n.d. | n.d. | n.d. |
1n.d.: not detected; 2Normal Fat-Soybean oil (NF-So), diet containing 4.0% soybean oil (SO); 3High Fat-Control Butter (HF-Cb), diet containing 21.7% control butter and 2.3% SO; 4High CLA Butter (HF-CLAb), diet containing 21.7% butter naturally enriched in cis-9, trans-11 CLA and 2.3% SO; 5High Fat-Soybean oil (HF-So), diet containing 24.0% SO.