Table 1.
Phenotypic measures in NBD-treated and untreated GRMD and normal dogs
Test | Normal (Untreated) (n = 8) | Normal (NBD-treated) (n = 3) | GRMD (Untreated) (n = 10) | GRMD (NBD-treated) (n = 6) | ||||
---|---|---|---|---|---|---|---|---|
Age (months) | Age (months) | Age (months) | Age (months) | |||||
3 | 6 | 2 | 6 | 3 | 6 | 2 | 6 | |
Body Mass (kg) | 8.46 ± 0.632* | 17.80 ± 0.441***,2*** | 4.99 ± 1.83 | 21.10 ± 3.151* | 7.36 ± 1.29 | 13.40 ± 2.201*** | 3.60 ± 0.27 | 12.70 ± 2.811** |
Absolute Tetanic Flexion Force (N) | 9.51 ± 1.072*** | 23.19 ± 2.201***,2*** | 4.52 ± 1.82 | 25.75 ± 2.181** | 3.85 ± 1.13 | 7.85 ± 1.981*** | 2.05 ± 0.58 | 6.79 ± 1.131*** |
Body-mass Corrected Tetanic Flexion Force (N/kg) | 1.12 ± 0.102*** | 1.30 ± 0.131*,2*** | 0.90 ± 0.042** | 1.24 ± 0.19 | 0.53 ± 0.14 | 0.62 ± 0.18 | 0.57 ± 0.15 | 0.55 ± 0.13 |
Absolute Tetanic Flexion Torque (Nm) | 1.08 ± 0.142*** | 3.22 ± 0.351***,2*** | 0.42 ± 0.24 | 3.65 ± 0.431** | 0.43 ± 0.14 | 1.03 ± 0.231*** | 0.17 ± 0.06 | 0.89 ± 0.181*** |
Body-mass Corrected Tetanic Flexion Torque (Nm/kg) | 0.13 ± 0.012*** | 0.18 ± 0.021***,2*** | 0.08 ± 0.02 | 0.17 ± 0.02 | 0.06 ± 0.02 | 0.08 ± 0.02 | 0.05 ± 0.01 | 0.07 ± 0.021* |
Absolute Tetanic Extension Force (N) | 22.56 ± 2.762*** | 46.43 ± 8.441***,2*** | 13.46 ± 3.26 | 42.17 ± 7.461* | 12.73 ± 2.12 | 18.47 ± 10.11 | 7.50 ± 2.09 | 34.30 ± 9.651**,2* |
Body-mass Corrected Tetanic Extension Force (N/kg) | 2.68 ± 0.382** | 2.61 ± 0.492** | 2.88 ± 1.10 | 2.00 ± 0.22 | 1.80 ± 0.52 | 1.56 ± 0.92 | 2.11 ± 0.70 | 2.70 ± 0.652* |
Absolute Tetanic Extension Torque (Nm) | 2.55 ± 0.372*** | 6.44 ± 1.241***,2*** | 1.20 ± 0.41 | 6.02 ± 1.381* | 1.42 ± 0.23 | 2.46 ± 1.28 | 0.62 ± 0.16 | 4.55 ± 1.491**,2* |
Body-mass Corrected Tetanic Extension Torque (Nm/kg) | 0.30 ± 0.042*** | 0.36 ± 0.072*** | 0.25 ± 0.08 | 0.28 ± 0.04 | 0.20 ± 0.05 | 0.18 ± 0.10 | 0.21 ± 0.07 | 0.36 ± 0.081*,2** |
ECD (%; 1-10) | 13.68 ± 3.97 | 8.29 ± 2.881*,2** | 13.36 ± 2.65 | 14.47 ± 7.31 | 13.97 ± 10.52 | 28.96 ± 18.07 | 16.12 ± 5.88 | 32.04 ± 13.46 |
ECD (%; 1-30) | 26.80 ± 5.922* | 17.86 ± 5.201*,2*** | 33.44 ± 10.3 | 23.25 ± 17.4 | 36.51 ± 11.49 | 54.30 ± 14.731* | 38.67 ± 10.34 | 54.48 ± 13.64 |
TTJ Angle (o) | 167.9 ± 3.942*** | 160.9 ± 3.401**,2* | 161.7 ± 7.64 | 158.3 ± 2.08 | 159.40 ± 3.72 | 151.30 ± 10.19 | 157.00 ± 9.80 | 160.17 ± 8.84 |
Hip (Resting) | 99.50 ± 5.10 | 104.4 ± 5.76 | 99.33 ± 1.15 | 103.0 ± 11.4 | 103.4 ± 8.96^ | 109.6 ± 8.02 | 103.7 ± 2.94 | 100.2 ± 6.012* |
Maximum Hip Flexion (o) | 55.50 ± 8.86 | 59.00 ± 12.60 | 48.67 ± 7.77 | 53.33 ± 10.12 | 54.40 ± 7.50^ | 68.60 ± 16.15 | 44.67 ± 5.47 | 46.33 ± 3.502** |
Pelvic Angle (o) | 43.75 ± 5.12 | 36.14 ± 5.982** | 38.33 ± 1.53 | 40.33 ± 5.03 | 40.00# | 48.30 ± 5.54 | 41.00 ± 6.81 | 36.83 ± 5.122** |
CS Circumference (mm/kg) | NA | 2.09 ± 0.402*** | NA | 2.30 ± 0.49 | NA | 4.03 ± 1.04 | NA | 3.67 ± 0.65 |
Functional studies, chiefly TTJ tetanic force and pelvic limb joint angles, were assessed to demonstrate therapeutic benefit of NBD in GRMD dogs and potential effects in wild type dogs. N = Newtons; Nm = Newton meters; ECD = Eccentric contraction deficit; CS = Cranial Sartorius; TTJ = tibiotarsal joint.
^Data for only five GRMD dogs available at 3 mos.
#Data for only one GRMD dog available at 3 mos.
1Significantly different from 2/3 to 6 mos, same genotype; *p < 0.05, **p < 0.01; ***p < 0.001.
2Significantly different from GRMD natural history; *p < 0.05, **p < 0.01; ***p < 0.001.