| Reagent | Volume |
| PBS without Ca2+ or Mg2+ | 4.75 ml |
| Potassium ferricyanide (0.2 M) | 100 μl |
| Potassium ferrocyanide (0.2 M) | 100 μl |
| Magnesium chloride (1 M) | 20 μl |
| X-gal (50 mg/ml) | 40 μl |
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