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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Cell Microbiol. 2014 Jan 24;16(7):1053–1067. doi: 10.1111/cmi.12257

Fig. 4.

Fig. 4

saa mediates systemic neutrophil migration in response to the microbiota. Comparisons of 6dpf GF and CONVD Tg(mpx:GFP) zebrafish injected with either standard control (Ctrl MO) or a morpholino targeting saa (saa MO). A. Images of whole live 6dpf Tg(mpx:GFP) zebrafish show GFP(+) neutrophil localization including increased concentration of neutrophils in the kidney (white arrow) and intestine (white arrow head) in CONVD Ctrl MO and saa MO zebrafish. B. Neutrophil units quantified by GFP densitometry from whole animal images similar to those shown in A. C. qRT-PCR for mpx mRNA in sorted neutrophils. qRT-PCR for ncf1 (D) and il1b (E) mRNA in whole 6dpf zebrafish. Quantification of neutrophil velocity (F) and meandering index (G) in the CHT, intestine (Int), and fin (calculated from 7–29 neutrophils per tissue per condition). Student's t-test P-values are indicated: a, P < 0.05 compared with GF condition in same genotype; b, P < 0.05 compared with Ctrl MO in same microbial condition. See also Movies S3–S6.