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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Cell Microbiol. 2014 Jan 24;16(7):1053–1067. doi: 10.1111/cmi.12257

Fig. 5.

Fig. 5

SAA stimulation of a zebrafish cell line results in activation of the canonical NF-κB pathway and induces expression of NF-κB target genes. A. Western blot of zebrafish PAC-2 cells shows rapid phosphorylation of IκBα proteins after LPS (10 μg ml−1) or SAA (4 μM) stimulation. B. Zebrafish PAC-2 cells transfected with ikbaa-luciferase gene reporter (pikbaa:Luc) show increased luciferase activity upon stimulation with LPS (10 μg ml−1) or SAA (1, 4 μM). C and D. qRT-PCR using primers for mmp9 and ikbaa, predicted NF-κB target genes, demonstrate induction upon stimulation of PAC-2 cells with LPS (10 μg ml−1) or SAA (1, 4 μM) normalized to 18S ribosomal RNA [rRNA]). Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.