Fig. 2. Single reaction monitoring (SRM) LC-MS chromatogram for m/z transition 299–152 monitoring formation of O 6-pyridyloxybutyl-guanine (4, Scheme 4) adducts from biocolloid reactors (enzyme color code on bottom) featuring (a) human organ microsomes, (b) cyt P450 supersomes; (c) and (d) are total DNA adducts found (pmol {μg of protein}–1 {mM of NNK}–1) after reactions with 150 μM NNK at pH = 7.4 for 18 h, (c) human organ microsomes, (d) cyt P450 supersomes.
