Fig. 3. DNA damage rates and amounts of adducts from 2-AAF. Top panel – relative DNA damage rate ({μg of protein}^–1 s^–1 mM^–1) from ECL arrays exposed to 250 μM of 2-AAF in pH = 7.4 for (a) human organ tissue fractions, (b) cyt P450 supersomes. Bottom panel – total amount of DNA adducts found (pmol {μg of protein}^–1 {mM of 2-AAF}^–1) after reactions with 250 μM 2-AAF at pH = 7.4 for 4 h, (c) human organ microsomes, (d) cyt P450 supersomes. The ECL images and single reaction monitoring LC-MS/MS chromatograms are in ESI file (Fig. S4 and S7†).