Fig. 4. DNA damage rates and adduct amounts from styrene. Top panel – relative DNA damage rate ({μg of protein}^–1 s^–1 mM^–1) from ECL arrays exposed to 1 mM of styrene in pH = 7.4 for (a) human organ tissue enzymes, (b) cyt P450 supersomes. Bottom panel – total amount of DNA adducts found (pmol {μg of protein }^–1 {mM of styrene}^–1) after reactions with 1 mM of styrene at pH = 7.4 for 1 h, (c) human organ microsomes, (d) cyt P450 supersomes. The ECL images and single reaction monitoring LC-MS chromatograms are in ESI file (Fig S5 and S8†).